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ITEA  Der f 1 ELISA Self-build Kit (Biotin-labeled)

ITEA Der f 1 ELISA Self-build Kit (Biotin-labeled)

Item # Pack Size Storage
1-DF1-002 For 3 plates 2-8°C

This kit consists of the following antibodies and standard solutions required for measuring Japanese cedar pollen allergen (Der f 1) by sandwich ELISA.
An optional Streptavidin-HRP + TMB substrate solution set is also available.

  • ■ Key Benefits

    ・High specificity: Der f 1 specific, having no cross reactivity with Der f 2
    ・Can be performed at room temperature (no incubator required)
    ・Allows use according to plate requirements, minimizing reagent waste and saving space

  • ■ Usage examples

    ・Measurement of Der f 1 in house dust
    ・Measurement of Der f 1 in bedding
    ・Measurement of Der f 1 in foods
    ・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)
    ・Measurement of indoor Der 1 under the School Health and Safety Act

  • ■ Kit Contents

    Component Pack size
    Capture Anti-Der f 1 monoclonal antibody for coating1 (dissolved in Tris-Glycine-HCl buffer containing 50% glycerol)
    Der f 1 Standard2 (lyophilized)
    Biotin-labeled anti-Der f 1 monoclonal antibody1 (dissolved in Tris-HCl buffer containing 50% glycerol)
  • ■ Performance

    Measurement range 0.23-30 ng/ml
    Detection limit
    Curve fit 4-parameter logistic, or cubic formula recommended
  • ■ Standard curve example

    Standard curve example
  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.