| Item # | Pack Size | Storage |
|---|---|---|
| 1-OVA-001 | 96 wells/kit | Store at 2-8℃ |
ELISA kit for quantification of OVA（Ovalbumin） from chickens. The pre-blocked and coated microplates with capture antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement. It is useful for experiments on OVA kinetics in vivo.
・Quick measurement: test results in 2 hours and 15 minutes
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Quantification of OVA reagents for experiment
・Evaluation of transfected OVA expression
・Determination of OVA concentrations in blood and milk
| Component | Pack size |
|---|---|
| Capture antibody coated microplates | 2 packs(8 wells ×6 strips/pack) |
| OVA Standard (lyophilized) | 2 for 4 measurements |
| Enzyme-labeled anti-OVA antibody | 12 mL × 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution | 12 mL × 1 |
| Stop solution(0.5M sulfuric acid) | 12 mL × 1 |
| Dilute solution (for sample and reagent) | 30 mL × 2 |
| Wash solution (20x concentrated) | 30 mL × 1 (for 600 mL) |
| Microplate seal | 3 |
| Measurement range | 0.78 - 50 ng/mL |
|---|---|
| Detection limit | |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜3％ |
|---|---|
| Between-day imprecision | CV ＜14％ |
| Intermediate precision | CV ＜14％ |
OVA sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
| Sample | Dilution ratio | Recovery rate（%） | |
|---|---|---|---|
| Normal serum | Mouse | 1:10 | 96.5 |
| Rat | 1:10 | 97.6 | |
| Human | 1:10 | 94.5 | |
| Rabbit | 1:10 | 103.5 | |
| Dog | 1:10 | 103.1 | |
| Bovine fetus | 1:10 | 105.2 | |
| cell culture medium (10% bovine fetus serum added) |
RPMI | 1:10 | 99.5 |
| DMEM | 1:10 | 103.7 | |
Each serum and cell culture mediam (RPMI and DMEM with 10% bovine fetus serum) were used as samples.
It was shown that dilution of each sample by 10 times or more was sufficient to measure OVA accurately.
＜ Measurement of OVA expressed in cell lines＞
1) Shimizu K, et al. Vaccination with Antigen-Transfected, NKT Cell Ligand–Loaded, Human Cells Elicits Robust In Situ Immune Responses by Dendritic Cells. Cancer research 2013; 73(1): 62-73. [PubMed]
2) Shimizu K, et al. Systemic DC Activation Modulates the Tumor Microenvironment and Shapes the Long-Lived Tumor-Specific Memory Mediated by CD8+ T Cells. Cancer Research 2016; 76(13): 3756-3766. [Pubmed]
3) Chang C, et al. Stable Immune Response Induced by Intradermal DNA Vaccination by a Novel Needleless Pyro-Drive Jet Injector. AAPS PharmSciTech 2019; 21: 19. [Pubmed]
4) Shimizu K, et al. Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN. Front Immunol 2019; 10: 1450. [Pubmed]
＜Measurement of OVA concentration in blood＞
1) Sae-Wong C, et al. Topical skin treatment with Fab fragments of an allergen-specific IgG1 monoclonal antibody suppresses allergen-induced atopic dermatitis-like skin Lesions in mice. European journal of pharmacology 2016; 779: 131-137. [Pubmed]
2) Tanabe K, et al. Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model. Scientific reports 2015; 5: 14424. [Pubmed]
3) Yamamoto T, et al. Oral tolerance induced by transfer of food antigens via breast milk of allergic mothers prevents offspring from developing allergic symptoms in a mouse food allergy model. Clinical and Developmental Immunology 2012; 2012: 721085.
＜Literature on this kit overview＞
Kurata K and Shirai S. [Development of an enzyme-linked immunosorbent assay kit for measuring ovalbumin concentration in biomaterials]. The Allergy in practice 2012; 32: 177-180. (Japanese)
All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
