| Item # | Pack Size | Storage |
|---|---|---|
| 1-DFH-001 | 96 wells/kit | Store at 2-8℃ |
This is a high sensitive ELISA kit which is available for measurement of Der f 1 at low concentration, 31.2-2000 pg/mL. It is suitable for measurement of a very small amount of Der f 1 in air or house dust, inspection for mite contamination in food ingredients, and verification of allergen reduction effect.
・High sensitivity: the detection limit is 31.2 pg/mL
・Mesurement with an absorbance plate reader (450 nm)
・Quick measurement: test results in 3 hours and 45 minutes
・High specificity: having no cross reactivity with Der p 1
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Measurement of Der f 1 in house dust
・Measurement of Der f 1 in bedding
・Measurement of Der f 1 in foods
・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)
| Component | Pack size |
|---|---|
| Capture antibody coated microplates | 2 packs(8 wells ×6 strips/pack) |
| Der f 1 Standard (lyophilized) | 2 for 4 measurements |
| Biotin-labeled anti-Der f 1 antibody | 120 µL × 1 |
| Enzyme-labeled streptavidin | 120 µL × 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution | 12 mL × 1 |
| Stop solution(0.5M sulfuric acid) | 12 mL × 1 |
| Dilute solution (for sample and reagent) | 30 mL × 2 |
| Wash solution (20x concentrated) | 30 mL × 1 (for 600 mL) |
| Microplate seal | 3 |
| Measurement range | 31.2 - 2000 pg/mL |
|---|---|
| Detection limit | |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV < 5％ |
|---|---|
| Intermediate precision | CV < 5％ |
Six samples adjusted to low, medium, and high concentration ranges within the standard curve were independently measured four times and calculated.
Der f 1 was specifically measured and Der p 1（0.4、4、40、400、4000 ng/mL) was no cross with in this assay.
All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
