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ITEA  Der f 2 ELISA Self-build Kit (Biotin-labeled)

ITEA Der f 2 ELISA Self-build Kit (Biotin-labeled)

Item # Pack Size Storage
1-DF2-002 For 3 plates 2-8°C

This kit consists of the following antibodies and standard solutions required for measuring house dust mites (Dermatophagoides farinae)(Der f 2) by sandwich ELISA.
* Der f 2 concentration in samples including Der f 2 and Der p 2 cannot be precisely quantified with this kit which has cross-reactivity to Der f 2 and Der p 2 both, and a standard for Der f 2 were used in allergen quantification.

  • ■ Usage examples

    ・Measurement of Der f 2 in house dust
    ・Measurement of Der f 2 in bedding
    ・Measurement of Der f 2 in foods
    ・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)

  • ■ Kit Contents

    Component Pack size
    Capture Anti-Der f 2 monoclonal antibody for coating1 (dissolved in Tris-Glycine-HCl buffer containing 50% glycerol)
    Der f 2 Standard2 (lyophilized)
    Biotin-labeled anti-Der f 2 monoclonal antibody1 (dissolved in Tris-HCl buffer containing 50% glycerol)
  • ■ Performance

    Measurement range 0.08-10 ng/mL
    Detection limit
    Curve fit 4-parameter logistic, or cubic formula recommended
  • ■ Standard curve example

    Standard curve example
  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.