| Item # | Pack Size | Storage |
|---|---|---|
| 1-DF1-001 | 96wells/kit | Store at 2-8℃ |
ELISA kit for quantification of the group 1 allergen from house dust mites （Dermatophagoides farinae）, Der f 1. The pre-blocked and coated microplates with capture antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement.
・Quick measurement: test results in 2 hours and 15 minutes
・High specificity: having no cross reactivity with Der p 1
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Measurement of Der f 1 in house dust
・Measurement of Der f 1 in bedding
・Measurement of Der f 1 in foods
・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)
・Measurement of indoor Der 1 under the School Health and Safety Act
| Component | Pack size |
|---|---|
| Capture antibody coated microplates | 2packs(8 wells ×6 strips/pack) |
| Der f 1 Standard (lyophilized) | 2 for 4 measurements |
| Enzyme-labeled anti-Der f 1 antibody | 12 mL × 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution | 12 mL × 1 |
| Stop solution(0.5M sulfuric acid) | 12 mL × 1 |
| Dilute solution (for sample and reagent) | 30 mL × 2 |
| Wash solution (20x concentrated) | 30 mL × 1 (for 600 mL) |
| Microplate seal | 3 |
| Measurement range | 0.23 - 30 ng/mL |
|---|---|
| Detection limit | |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜4％ |
|---|---|
| Between-day imprecision | CV ＜8％ |
| Intermediate precision | CV ＜9％ |
Der f 1 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
Der f 1 was specifically measured and Der p 1（0.4、4、40、400、4000 ng/mL) was no cross with in this assay.
1) Kitazawa H, et al. Egg antigen was more abundant than mite antigen in children’s bedding: Findings of the pilot study of the Japan Environment and Children’s Study (JECS). Allergology International 2019; 68: 391–393.[Pubmed]
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All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
