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ITEA Cry j 1 ELISA Self-build Kit (Biotin-labeled)

ITEA Cry j 1 ELISA Self-build Kit (Biotin-labeled)

Item # Pack Size Storage
1-CJ1-002 For 3 plates 2-8°C

This kit consists of the following antibodies and standard solutions required for measuring Japanese cedar pollen allergen (Cry j 1) by sandwich ELISA.

  • ■ Key Benefits

    ・High specificity: Cry j 1 specific, having no cross reactivity with Cry j 2
    ・Can be performed at room temperature (no incubator required)
    ・Allows use according to plate requirements, minimizing reagent waste and saving space

  • ■ Usage examples

    ・Control of the pollen lots
    ・Measurement of Cry j 1 in house dust
    ・Measurement of Cry j 1 in the air
    ・Evaluation and control of Cry j 1 concetration in a chamber that has been scattered
    ・Development and evaluation of products against the pollen (filters, air purifiers, cleaners and so on)

  • ■ Kit Contents

    Component Pack size
    Capture Anti-Cry j 1 monoclonal antibody for coating (S10)1 (dissolved in Tris-Glycine-HCl buffer containing 50% glycerol)
    Cry j 1 Standard2 (lyophilized)
    Biotin-labeled anti-Cry j 1 monoclonal antibody (S131)1 (dissolved in Tris-HCl buffer containing 50% glycerol)
  • ■ Performance

    Measurement range 0.16–10 ng/ml
    Detection limit
    Curve fit 4-parameter logistic, or cubic formula recommended
  • ■ Standard curve example

    Standard curve example
  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.