| Item # | Pack Size | Storage |
|---|---|---|
| 1-CJ1-001 | 96 wells/kit | Store at 2-8℃ |
ELISA kit for quantification of the major allergen from (Cryptomeria japonica), Cry j 1.
The pre-blocked and coated microplates with capture antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement.
・Quick measurement: test results in 2 hours and 15 minutes
・High specificity: Cry j 1 specific, having no cross reactivity with Cry j 2
・Can be performed at room temperature (no incubator required)
・Easy to use: microplates that can be used without washing after opening
・Compact package
・Control of the pollen lots
・Measurement of Cry j 1 in house dust
・Measurement of Cry j 1 in the air
・Evaluation and control of Cry j 1 concetration in a chamber that has been scattered
・Development and evaluation of products against the pollen (filters, air purifiers, cleaners and so on)
| Component | Pack size |
|---|---|
| Capture antibody coated microplates | 2 packs(8 wells ×6 strips/pack) |
| Cry j 1 Standard (lyophilized) | 2 for 4 mesurements |
| Enzyme-labeled anti-Cry j 1 antibody | 12 mL × 1 |
| TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution | 12 mL × 1 |
| Stop solution(0.5M surfuric acid) | 12 mL × 1 |
| Dilute solution (for sample and regent) | 30 mL × 2 |
| Wash solution (20x concentrated) | 30 mL × 1 (for 600 mL) |
| Microplate seal | 3 |
| Measurement range | 0.16 - 10 ng/mL |
|---|---|
| Detection limit | |
| Curve fit | 4-parameter logistic, or cubic formula recommended |
| Within-day imprecision | CV ＜3％ |
|---|---|
| Between-day imprecision | CV ＜7％ |
| Intermediate precision | CV ＜8％ |
Cry j 1 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.
Cry j 1 was specifically measured and Cry j 2 (0.1、1、10、100 ng/mL) was no cross with in this assay.
<Literature on the monoclonal antibodies used in this kit>
1) Watanabe M, et al. [An enzyme-linked immunosorbent assay for the quantitation of the major allergen from Japanese cedar (Cryptomeria japonica) pollen, Cry j I, using monoclonal antibodies]. Arerugi 1992; 41(11): 1535-1539. (Japanese) [J-STAGE]
2) Takahashi Y, et al. [Protracted (lasting) presence of Japanese cedar pollen allergen (Cry j I) in house dust]. Arerugi 1994; 43(2): 97-100. (Japanese) [J-STAGE]
3) Sakaguchi M, et al. Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis. Immunology 1997; 91(2): 161-166. [PubMed]
4) Midoro-Horiuti T, et al. (2006) Structural basis for epitope sharing between group 1 allergens of cedar pollen. Mol Immunol 2006; 43: 509–518. [PubMed]
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All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.
